Immunoelectron Microscopic Localization Of Neural Cell Adhesion Molecules L1, N-cam, And Myelin-associated Glycoprotein In Regenerating Adult Mouse Sciatic Nerve
After a penetrating lesion within the central nervous system, astrocytes enlarge, divide, and participate in creating an environment that adversely affects neuronal regeneration. We have lately shown that the neural cell adhesion molecule (N-CAM) partially inhibits the division of early postnatal rat astrocytes in vitro. Animals had been lesioned within the cerebral cortex, hippocampus, or striatum with a Hamilton syringe and needle at outlined stereotaxic positions. On one facet, the lesions were concomitantly infused with N-CAM or with one of the N-CAM-related molecules. As a control, a peptide of the identical composition because the N-CAM decapeptide but of random sequence was infused on the contralateral side of the brain. We consistently found that the population of dividing astrocytes was significantly smaller on the facet by which N-CAM or one of…
